基因编辑文库设计和探针合成服务

2016-07-05

CRISPR/Cas9系统介导的基因定点编辑技术,可实现由sgRNA指导Cas9核酸酶对靶基因的DNA进行定点修饰,其特异性依赖于sgRNA与靶标位点通过碱基互补配对,并且靶标位点必须含有PAM序列(NGG)。

 

尽管目前有很多sgRNA设计软件,但实现高通量快速全基因组设计sgRNA的程序不多,宇玫博开发的拥有自主知识产权“高通量sgRNA文库设计软件”,该软件能针对任意物种,任意来源的序列设计sgRNA,可用于构建sgRNA表达载体用于基因组编辑实验,同时可用于设计sgRNA文库。

 

服务内容:

    1, 高通量sgRNA文库设计;

    2, sgRNA文库探针Oligo Pools合成;

          每个pool可以提供12,000 到90,000 不同的oligo序列.

    3, sgRNA文库慢病毒包装服务;

参考文章:

"Large-scale de novo DNA synthesis: technologies and applications," by Sriram Kosuri and George M. Church, Nature Methods, May, 2014, Vol. 11, No. 5, pp. 499–507.

"Genetic Screens in Human Cells Using the CRISPR/Cas9 System," by Tim Wang, Jenny J. Wei, David M. Sabatini, and Eric S. Lander, Science, Jan. 3, 2014, Vol. 343, no. 6166, pp. 80-84.

"Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells", by Ophir Shalem, Neville E. Sanjana, Ella Hartenian, Xi Shi, David A. Scott, Tarjei Mikkelson, Dirk Heckl, Benjamin L. Ebert, David E. Root, John G. Doench, Feng Zhang, Science, 3 January 2014, Vol. 343, no. 6166, pp. 84-87.

"Robust Chemical Preservation of Digital Information on DNA in Silica with Error-Correcting Codes," by Grass, R. N., Heckel, R., Puddu, M., Paunescu, D. and Stark, W. J. (2015). Angew. Chem. Int. Ed., 54: 2552–2555.

下一篇:化学合成服务
本网站由阿里云提供云计算及安全服务