实验性自身免疫性脑脊髓炎模型 (EAE)

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  • 实验方案:

    1. C57小鼠适应性生长后,尾静脉注射PT, 200ul只;

    2. 背部皮下注射MOG-CFA,200ul只;

    3. 48小时后再次注射PT, 同等剂量;

    4. EAE疾病通常在10-14天开始发病;

    5. 发病后每天观察老鼠状态,评分等。



    Brief protocol of EAE model:

    1. Put mice under irradiation of infrared light, which leads to vasodilation, and it will make tail vein injection PT much easier. 

    2. Pertussis toxin i.v. injection, 200ulmouse 

    3. 0.6% Pentobarbital Sodium was used as Anaesthesia.200-250μl each mouse 

    4. After anaesthesia, Hypodermic inject emulsified MOG, 200μl each mouse. 

    5. After 48h, do the tail vein injection the second time.

    6. Disease usually began to onset around day 10-14, clinical score will be recorded daily.